ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (5): 757-762.doi: 10.11843/j.issn.0366-6964.2014.05.011

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Construction of Tetracycline-inducible Expression System of H1foo Gene in Mouse

LI Jian, NING Jing, CHEN Xi-rui, SONG Cheng-yan, LEI An-min*   

  1. (Shaanxi Key Laboratory of Molecular Biology for Agriculture,Shaanxi Stem Cell Engineering and Technology Research Center,College of Veterinary Medicine,Northwest Agricultural and Forestry University,Yangling 712100, China)
  • Received:2013-10-14 Online:2014-05-23 Published:2014-05-23

Abstract:

The oocyte-specific linker histone H1foo is a subtype of linker histone H1,H1foo plays an essential role during meiosis and maturation of the oocytes,moreover,it also participates in epigenetic reprogramming.In order to investigate the function of H1foo further in somatic cells,we adopted the Tet gene expression system,The H1foo gene and the promoter of Tet-On regulatory region were amplified by PCR and inserted into pVenus to generate the recombinant vector pVenus-Tet-CMV-H1foo through double digestion and ligation.Restriction enzyme analysis and DNA sequencing showed that the sequence of the recombinant plasmid was correct and the fragments were properly connected.This Tetracycline-inducible mouse H1foo eukaryotic expression system will allow us to examine the role of H1foo at specific time points better during the somatic cell cycle.

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